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KMID : 0379119900180040225
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Korean Journal of Mycology
1990 Volume.18 No. 4 p.225 ~ p.232
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Inhibitory Conditions of Asexual Development and their Application for the Screening of Mutants Defective in Sexual Development
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이영훈/Lee, Young Hoon
채건상/한동민/장광엽/한유정/장승환/Chae, Keon Sang/Han, Dong Min/Jahng, Kwang Yeop/Han, Yoo Jeoung/Jahng, Seung Hwan
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Abstract
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In order to find an useful condition under which the mutants defective in sexual development could be isolated, the effects of several cultural conditions on the developments of Aspergillus nidulans were examined. Among several conditions found to restrict the asexual sporulation but enhance the sexual process, the interference of aeration by sealing the plates with sealing film was the most useful one for the purpose of mutant isolation. Sealing at any time before 20 hours from inoculation prevented both sexual and asexual process. When the seal was removed after 24 hours, however, the mycelia developed only to sexual organs. Using this properity, the early morphogenic process of sexual development could be easily observed and a number of mutants that showed some defects in the process could be isolated. The mutants were divided into 3 groups, NSD (never in sexual development), BSD (block in sexual development) and ASD (abnormal in sexual development). NSD mutants never produced either the Hu¨lle cells or cleistothecia and some produced the asexual organs even when the aeration was restricted. BSD mutants were blocked in the process of Hu¨lle cell, cleistothecia, crozier, asci or ascospore formation. ASD mutants had defects in the amount of cleistothecia, time of cleistothecial maturation or color of ascospores.
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KEYWORD
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